As it is known to all that qualitatively matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) represents high sensitivity and robustness for the structural characterization of glycosylated compounds and can also be used in a high-throughput manner. It is also the preference for singly charged ion formation that makes MALDI a better choice than electrospray ionization for profiling mixtures of N-glycans. The substrates of N-Glycan profiling are permethylated glycans. That is to say, n-glycan profiling by enzymatic deglycosylation using Peptide N-glycosidase A or F is analyzed after permethylation.
Citation InformationMelissa George. "n-glycan profiling" (2017)
Available at: http://works.bepress.com/creativeproteomics/3/