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Human Brain Microvascular Endothelial Cells
(2005)
  • Betty Cummings
Abstract

Human Brain Microvascular Endothelial Cells from Creative Bioarray are isolated from human brain tissue. Human Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Keywords
  • Human Brain Microvascular Endothelial Cells,
  • primary cells,
  • cells
Publication Date
Summer December 18, 2005
Citation Information
Betty Cummings. "Human Brain Microvascular Endothelial Cells" (2005)
Available at: http://works.bepress.com/creative-bioarray/4/