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Article
Biodiversity and Emerging Biogeography of the Neutrophilic Iron-Oxidizing Zetaproteobacteria
Applied and Environmental Microbiology
  • Sean M. McAllister
  • Richard E. Davis
  • Joyce M. McBeth
  • Bradley M. Tebo
  • David Emerson
  • Craig L. Moyer, Western Washington University
Document Type
Article
Publication Date
1-1-2011
Disciplines
Abstract

Members of the neutrophilic iron-oxidizing candidate class Zetaproteobacteria have predominantly been found at sites of microbially mediated iron oxidation in marine environments around the Pacific Ocean. Eighty-four full-length (>1,400-bp) and 48 partial-length Zetaproteobacteria small-subunit (SSU) rRNA gene sequences from five novel clone libraries, one novel Zetaproteobacteria isolate, and the GenBank database were analyzed to assess the biodiversity of this burgeoning class of the Proteobacteria and to investigate its biogeography between three major sampling regions in the Pacific Ocean: Loihi Seamount, the Southern Mariana Trough, and the Tonga Arc. Sequences were grouped into operational taxonomic units (OTUs) on the basis of a 97% minimum similarity. Of the 28 OTUs detected, 13 were found to be endemic to one of the three main sampling regions and 2 were ubiquitous throughout the Pacific Ocean. Additionally, two deeply rooted OTUs that potentially dominate communities of iron oxidizers originating in the deep subsurface were identified. Spatial autocorrelation analysis and analysis of molecular variance (AMOVA) showed that geographic distance played a significant role in the distribution of Zetaproteobacteria biodiversity, whereas environmental parameters, such as temperature, pH, or total Fe concentration, did not have a significant effect. These results, detected using the coarse resolution of the SSU rRNA gene, indicate that the Zetaproteobacteria have a strong biogeographic signal.

Subjects - Topical (LCSH)
Biodiversity--Monitoring--Pacific Ocean; Proteobacteria--Pacific Ocean; Iron bacteria--Pacific Ocean; Iron--Oxidation--Pacific Ocean
Geographic Coverage
Pacific Ocean
Genre/Form
articles
Type
Text
Language
English
Format
application/pdf
Citation Information
Sean M. McAllister, Richard E. Davis, Joyce M. McBeth, Bradley M. Tebo, et al.. "Biodiversity and Emerging Biogeography of the Neutrophilic Iron-Oxidizing Zetaproteobacteria" Applied and Environmental Microbiology Vol. 77 Iss. 15 (2011) p. 5445 - 5457
Available at: http://works.bepress.com/craig_moyer/5/