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Pseudomonas Aeruginosa Strain MA01 Aerobically Metabolizes the Aminodinitrotoluenes Produced by 2,4,6-Trinitrotoluene Nitro Group Reduction
Canadian Journal of Microbiology
  • Mark A. Alvarez, Los Alamos National Laboratory, Los Alamos, New Mexico
  • Christopher Kitts, Los Alamos National Laboratory, Los Alamos, New Mexico
  • James L. Botsford, New Mexico State University, Las Cruces, NM
  • Pat J. Unkefer, Los Alamos National Laboratory, Los Alamos, New Mexico
Publication Date
11-1-1995
Abstract
Many microbes reduce the nitro substituents of 2,4,6-trinitrotoluene (TNT), producing aminodinitrotoluenes (ADNTs). These compounds are recalcitrant to further breakdown and are acutely toxic. In a search for organisms capable of metabolizing ADNTs, a bacterial strain was isolated for the ability to use 2-aminobenzoate (anthranilate) as sole C-source. This isolate, Pseudomonas aeruginosa MAO1, metabolized TNT by first reducing one nitro group to form either 2-amino-4,6-dinitrotoluene (2ADNT) or 4 -amino-2,6-dinitrotoluene(4ADNT). However, strain MA01 was distinct from other TNT-reducing organisms in that it transformed these compounds into highly polar metabolites through an 02-dependent process. Strain MAOl was able to cometabolize TNT, 2ADNT, and 4ADNT in the presence of a variety of carbon and energy sources. During aerobic cometabolism with succinate, 45% of uniformly ring-labeled [14C]TNT was transformed to highly polar compounds. Aerobic cometabolism of purified [14C]2ADNT and [14C]4ADNT with succinate as C-source produced similar amounts of these polar metabolites. During O2-limited cometabolism with succinate as C-source and nitrate as electron acceptor, less than 8% of the [14C]TNT was transformed to polar metabolites. Purified 2,6-diamino-4 -nitrotoluene was not metabolized, and while 2,4-diamino-6-nitrotoluenewas acetylated, the product (N-acetyl-2,4-diamino-6-nitrotoluene) was not further metabolized. Therefore, strain MA0l metabolized TNT by oxidation of the ADNTs and not by reduction the remaining nitro groups on the ADNTs.
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Citation Information
Mark A. Alvarez, Christopher Kitts, James L. Botsford and Pat J. Unkefer. "Pseudomonas Aeruginosa Strain MA01 Aerobically Metabolizes the Aminodinitrotoluenes Produced by 2,4,6-Trinitrotoluene Nitro Group Reduction" Canadian Journal of Microbiology Vol. 41 Iss. 11 (1995) p. 984 - 991
Available at: http://works.bepress.com/ckitts/21/