Skip to main content
Article
Removal of PCR Inhibitors From Soil DNA by Chemical Flocculation
Journal of Microbiological Methods
  • Michael D. Braid, Ciphergen Biosystems, Inc.
  • Laura M. Daniels, University of California - Davis
  • Christopher L. Kitts, California Polytechnic State University - San Luis Obispo
Publication Date
3-1-2003
Abstract
Extracting high-purity DNA directly from soil has become essential for the study of microorganisms in environmental samples. However, many soils contain compounds that inhibit enzymes involved in manipulating DNA. In this study, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process. The addition of AlNH4(SO4)2 during extraction significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield.
Disciplines
Citation Information
Michael D. Braid, Laura M. Daniels and Christopher L. Kitts. "Removal of PCR Inhibitors From Soil DNA by Chemical Flocculation" Journal of Microbiological Methods Vol. 52 Iss. 3 (2003) p. 389 - 393
Available at: http://works.bepress.com/ckitts/11/