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On-Chip Controlled Surfactant–DNA Coil–Globule Transition by Rapid Solvent Exchange Using Hydrodynamic Flow Focusing
Langmuir (2014)
  • Ciprian Iliescu
  • Cătălin Mărculescu
  • Shrinivas Venkataraman, Institute of Bioengineering and nanotechnology
  • Baptiste Languille
  • Hanry Yu
  • Guillaume Tresset
Abstract

This paper presents a microfluidic method for precise control of the size and polydispersity of surfactant−DNA nanoparticles. A mixture of surfactant and DNA dispersed in 35% ethanol is focused between two streams of pure water in a microfluidic channel. As a result, a rapid change of solvent quality takes place in the central stream, and the surfactant-bound DNA molecules undergo a fast coil−globule transition. By adjusting the concentrations of DNA and surfactant, fine-tuning of the nanoparticle size, down to a hydrodynamic diameter of 70 nm with a polydispersity index below 0.2, can be achieved with a good reproducibility.

Keywords
  • nanoparticles,
  • DNA-proteine interaction,
  • microfluidics
Disciplines
Publication Date
Fall November 1, 2014
Citation Information
Ciprian Iliescu, Cătălin Mărculescu, Shrinivas Venkataraman, Baptiste Languille, et al.. "On-Chip Controlled Surfactant–DNA Coil–Globule Transition by Rapid Solvent Exchange Using Hydrodynamic Flow Focusing" Langmuir Vol. 30 Iss. 44 (2014)
Available at: http://works.bepress.com/ciprian_iliescu/2/