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Localization of Label-Retaining Cells in Murine Vocal Fold Epithelium
Journal of Speech, Language & Hearing Research (2011)
  • Ciara Leydon, Sacred Heart University
  • Rebecca S Bartlett, University of Wisconsin - Madison
  • Drew A Roenneburg, University of Wisconsin - Madison
  • Susan L Thibeault, University of Wisconsin - Madison

Purpose: Epithelial homeostasis is critical for vocal fold health, yet little is known about the cells that support epithelial self-renewal. As a known characteristic of stem cells is that they are slowcycling in vivo, the purpose of this prospective controlled study was to identify and quantify slow-cycling cells or putative stem cells in murine vocal fold epithelium.

Method: Twelve mice were administered daily intraperitoneal injections of a nucleotide dye, bromodeoxyuridine (BrdU), over 7 consecutive days. Under this pulse-chase paradigm, slow-cycling cells retain the dye (label-retaining cells; LRCs) while more rapidly cycling cells lose dye to dilution during multiple cell divisions. The percentage of label-retaining cells (%LRCs) was calculated following a chase period of 2, 4, and 8 weeks postinjections.

Results: The %LRCs decreased significantly from 9.4% at 2 weeks to 3.1% at 8 weeks following injections (p < .05). No statistically significant differences in the quantity of BrdU-positive cells were measured between the anterior, mid-membranous, or cartilaginous regions of the vocal fold (p > .05).

Conclusion: These findings are consistent with the presence and first report of a small population of putative stem cells along the length of murine vocal fold epithelium.

  • larynx,
  • stratified squamous epithelium,
  • airway,
  • stem cells,
  • BrdU
Publication Date
August, 2011
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Citation Information
Ciara Leydon, Rebecca S Bartlett, Drew A Roenneburg and Susan L Thibeault. "Localization of Label-Retaining Cells in Murine Vocal Fold Epithelium" Journal of Speech, Language & Hearing Research Vol. 54 Iss. 4 (2011)
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