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Molecular basis of an inherited epilepsy
Neuron (2002)
  • Christoph Lossin, University of California, Davis
  • T. H. Rhodes, Vanderbilt University
  • C. Vanoye, Vanderbilt University
  • D. Wang, Vanderbilt University
  • Alfred L. George, Vanderbilt University
Abstract
Mutations in SCN1A, the gene encoding the brain voltage-gated sodium channel alpha1 subunit (NaV1.1), are associated with at least two forms of epilepsy, generalized epilepsy with febrile seizures plus (GEFS+) and severe myoclonic epilepsy of infancy (SMEI). We examined the functional properties of four GEFS+ alleles and one SMEI allele using whole-cell patch-clamp analysis of heterologously expressed recombinant human SCN1A. One previously reported GEFS+ mutation (I1656M) and an additional novel allele (R1657C), both affecting residues in a voltage-sensing S4 segment, exhibited a similar depolarizing shift in the voltage dependence of activation. Additionally, R1657C showed a 50% reduction in current density and accelerated recovery from slow inactivation. Unlike three other GEFS+ alleles that we recently characterized, neither R1657C nor I1656M gave rise to a persistent, noninactivating current. In contrast, two other GEFS+ mutations (A1685V and V1353L) and L986F, an SMEI-associated allele, exhibited complete loss of function. In conclusion, our data provide evidence for a wide spectrum of sodium channel dysfunction in familial epilepsy and demonstrate that both GEFS+ and SMEI can be associated with nonfunctional SCN1A alleles.
Publication Date
2002
Citation Information
Christoph Lossin, T. H. Rhodes, C. Vanoye, D. Wang, et al.. "Molecular basis of an inherited epilepsy" Neuron Vol. 37 (2002)
Available at: http://works.bepress.com/christoph_lossin/8/