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Heterogeneous rotational diffusion of a fluorescent probe in lipid monolayers
Structural Dynamics (2014)
  • Christina M Othon, Wesleyan University

The rotational correlation time of the lipid probe 1-palmitoyl-2-{6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC) is measured using fluorescence anisotropy for two lipid species. We measure the rotational diffusion in a monolayer of 1,2-Didecanoyl-sn-glycero-3-phosphocholine (DPPC) which displays a phase transition at room temperature from the liquid expanded to the liquid-condensed phase. The constant rotational diffusion of the probe throughout the phase transition reflects the measurement of dynamics in only the liquid-expanded phase. We contrast the dynamic changes during this phase coexistence to the continuous density increase observed in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at room temperature. We observe a non-exponential decay of the probe diffusion consistent with heterogeneity of the orientational dynamics.

  • Heterogeneous Dynamics,
  • Lipid Dynamics,
  • Membrane Structure,
  • diffusion,
  • rotational diffusion,
  • Time-resolved fluorescence anisotropy,
  • lipid probes
Publication Date
Summer August 27, 2014
Citation Information
Christina M Othon. "Heterogeneous rotational diffusion of a fluorescent probe in lipid monolayers" Structural Dynamics Vol. 1 (2014)
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