This complete research paper examines the impact of mastery learning homework on student academic motivation in a first year engineering course. Student learning in undergraduate engineering is most effective when it occurs both inside and outside of the classroom. For this reason, homework can be a useful tool for formative assessment. One approach to homework that has shown promise in increasing retention of course material is mastery learning. The mastery learning approach to education theorizes that any student can meet high standards for learning given the right circumstances. Specifically, a student that is learning for mastery should have personalized instruction, an appropriate amount of time, and the ability to persevere. Mastery learning approaches in engineering education have been proven to increase student learning without adding a significant amount of time and effort on the part of the instructor. In this study, a gamified online homework platform incorporates a mastery learning approach to gain insight into a student’s persistence and academic motivation for completing homework. This research seeks to answer the following research questions: 1) Which content areas do students struggle with more than others on an online mastery learning homework system? And 2) What relationship exists between the number of attempts on an online mastery learning homework system and students’ academic motivation? The number of attempts for mastery level completion across different content areas was collected alongside an academic motivation questionnaire. A one-way ANOVA was utilized to study the number of attempts for mastery level completion across different content areas as specified in research question one. The results of the academic motivation questionnaire were analyzed using Pearson correlations and independent samples T-tests. The results are presented with tests for statistical significance and Cohen’s D values. Results of this research are promising first steps in gaining a better understanding of how engineering students work through mastery learning homework, the impacts it can have on their academic motivation.