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Article
A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform
Biosensors
  • Nan Xiao, Iowa State University
  • Chao Wang, Iowa State University
  • Chenxu Yu, Iowa State University
Document Type
Article
Publication Date
9-13-2013
DOI
10.3390/bios3030312
Abstract

Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform. A self-referencing scheme was developed and implemented in which surface enhanced Raman spectroscopic (SERS) signatures of the targets were observed superimposed with the SERS signals of the Raman tags. The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration. The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.

Comments

This article is from Biosensors 3 (2013): 312–326, doi:10.3390/bios3030312.

Rights
© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Copyright Owner
The authors
Language
en
Date Available
2013-10-31
File Format
application/pdf
Citation Information
Nan Xiao, Chao Wang and Chenxu Yu. "A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform" Biosensors Vol. 3 Iss. 3 (2013) p. 312 - 326
Available at: http://works.bepress.com/chenxu_yu/6/