Nitrous oxide (N2O) is an important greenhouse gas and also an ozone-depleting substance that has both natural and anthropogenic sources. Large estimation uncertainty remains on the magnitude and spatiotemporal patterns of N2O fluxes and the key drivers of N2O production in the terrestrial biosphere. Some terrestrial biosphere models have been evolved to account for nitrogen processes and to show the capability to simulate N2O emissions from land ecosystems at the global scale, but large discrepancies exist among their estimates primarily because of inconsistent input datasets, simulation protocol, and model structure and parameterization schemes. Based on the consistent model input data and simulation protocol, the global N2O Model Intercomparison Project (NMIP) was initialized with 10 state-of-the-art terrestrial biosphere models that include nitrogen (N) cycling. Specific objectives of NMIP are to 1) unravel the major N cycling processes controlling N2O fluxes in each model and identify the uncertainty sources from model structure, input data, and parameters; 2) quantify the magnitude and spatial and temporal patterns of global and regional N2O fluxes from the preindustrial period (1860) to present and attribute the relative contributions of multiple environmental factors to N2O dynamics; and 3) provide a benchmarking estimate of N2O fluxes through synthesizing the multimodel simulation results and existing estimates from ground-based observations, inventories, and statistical and empirical extrapolations. This study provides detailed descriptions for the NMIP protocol, input data, model structure, and key parameters, along with preliminary simulation results. The global and regional N2O estimation derived from the NMIP is a key component of the global N2O budget synthesis activity jointly led by the Global Carbon Project and the International Nitrogen Initiative.