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Article
Radioimmunoassay for the Quantitative Determination of Hesperidin and Analysis of Its Distribution in Citrus Sinensis
Phytochemistry (1988)
  • Cecilia McIntosh, University of South Florida
  • Gary A. Barthe, University of South Florida
Abstract
A simple and sensitive radioimmunoassay (RIA) for the citrus flavanone hesperidin and other flavonoid 7-rutinosides is described. The assay utilizes antibodies raised against a hesperidin 4-O-carboxymethyl-oxime hapten and a tritiated radiotracer prepared by direct reduction of hesperidin with NaB[3-H]4. The detection limit of the assay is 0.2 ng/0.1 ml (0.3 pmol/0.1 ml and the measuring range extends to 10 ng/0.1 ml (16.4 pmol/0.1 ml). This assay is specific for flavonoid rutinosides, is characterized by a low coefficient of variation, and shows good correlation with HPLC for the quantification of hesperidin in oranges. The application of this method in determining the distribution of hesperidin in leaves, fruit, seeds, and seedlings of Citrus sinensis is also reported.
Keywords
  • flavonoidglycosides,
  • hesperidin,
  • radioimmunoassay,
  • Rutaceae,
  • rutinosides
Publication Date
1988
DOI
10.1016/0031-9422(88)80625-3
Citation Information
Cecilia McIntosh and Gary A. Barthe. "Radioimmunoassay for the Quantitative Determination of Hesperidin and Analysis of Its Distribution in Citrus Sinensis" Phytochemistry Vol. 27 Iss. 1 (1988) p. 249 - 254 ISSN: 0031-9422
Available at: http://works.bepress.com/cecilia-mcintosh/31/