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Article
Naringin and Limonin Production in Callus Cultures and Regenerated Shoots from Citrus sp.
Journal of Plant Physiology (1987)
  • G. A. Barthe, University of South Florida
  • P. S. Jourdan, University of South Florida
  • Cecilia McIntosh, University of South Florida
  • Richard L. Mansell, University of South Florida
Abstract
Limonin, limonoate A-ring monolactone, and naringin levels in Citrus paradisi Macf. and Citrus sinensis Osbeck were monitored during initiation of callus from hypocotyl sections and during shoot regeneration. The results show that the callus material contained substantially reduced levels of each of these compounds and that shoot development was accompanied by an increase in naringin and limonoate A-ring monolactone. Callus initiated from albedo explants showed a rapid decrease in limonin and naringin during the first three weeks of culture and by week 42 contained an average of 0.1 % of the original naringin and 0.06 % of the original limonin levels. Less than 1.5 % of the original naringin and limonin was lost to the culture medium. Limonin and naringin were still detectable in callus from different explant sources which had been in culture for over 3 years and a wide range of concentrations (over 10 fold) was found.
Keywords
  • callus culture initiation,
  • Citrus paradisi Macf.,
  • Citrus sinensis Osbeck,
  • limonin,
  • limonoate A-ring monolactone,
  • naringin,
  • radioimmunoassay
Publication Date
March 1, 1987
DOI
10.1016/S0176-1617(87)80041-X
Citation Information
G. A. Barthe, P. S. Jourdan, Cecilia McIntosh and Richard L. Mansell. "Naringin and Limonin Production in Callus Cultures and Regenerated Shoots from Citrus sp." Journal of Plant Physiology Vol. 127 Iss. 1 (1987) p. 55 - 65 ISSN: 0176-1617
Available at: http://works.bepress.com/cecilia-mcintosh/29/