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Article
n-Hexane toxicity in Jurkat T cells is mediated by reactive oxygen species
Archives of Toxicology (2008)
  • Catherine M. McDermott, University College Cork
  • Maria Hutch O'Donoghue, University College Cork
  • James J.A. Heffron, University College Cork
Abstract
Here we assess the role of reactive oxygen species (ROS) formation in the manifestation of n-hexane toxicity in Jurkat T-cells and the chemo-protective potential of the antioxidants epigallocatechin-3-gallate (EGCG) and thymoquinone (TQ) against n-hexane toxicity in vitro. n-Hexane is an important industrial solvent and ambient air pollutant. Sub-chronic exposure to n-hexane results in a concentration-dependent increase in ROS formation with a corresponding decrease in Jurkat T-cell proliferation. Results from time–course studies indicate that ROS formation plays a causal role in n-hexane induced alterations in Jurkat T-cell proliferation and membrane integrity. Treatment of cells with EGCG, at a concentration reached in plasma, reduced the ROS formation caused by exposure to n-hexane and inhibited the decrease in cell proliferation. Similar effects were obtained with TQ. Both EGCG and TQ significantly reduced n-hexane-induced LDH leakage to control levels. The combined results show that oxidative stress plays a role in the development of n-hexane toxicity.
Keywords
  • n-Hexane,
  • reactive oxygen species,
  • antioxidants,
  • membrane permeability
Publication Date
March 1, 2008
Publisher Statement
Citation only

McDermott, C., Hutch O'Donoghue, M., & Heffron, J.J.A (2008). n-Hexane toxicity in Jurkat T cells is mediated by reactive oxygen species. Archives in Toxicology, 82 (3), 165-171.

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© Copyright Springer- Verlag, 2008

Citation Information
Catherine M. McDermott, Maria Hutch O'Donoghue and James J.A. Heffron. "n-Hexane toxicity in Jurkat T cells is mediated by reactive oxygen species" Archives of Toxicology Vol. 82 Iss. 3 (2008)
Available at: http://works.bepress.com/catherine_mcdermott/3/