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Alterations in acetylcholine, PGE2 and IL6 release from urothelial cells following treatment with pyocyanin and lipopolysaccharide
oxicology in Vitro
  • Catherine M. McDermott, Bond University
  • Russ Chess-Williams, Bond University
  • Kylie A. Mills, Bond University
  • Sung-Hung Kang, Bond University
  • Stef Farr, Bond University
  • Gary D. Grant, Griffith University
  • Anthony V. Perkins, Griffith University
  • Andrew K. Davey, Griffith University
  • Shailendra Anoopkumar-Dukie, Griffith University
Date of this Version
1-1-2013
Document Type
Journal Article
Publication Details

Citation only

McDermott, C., Chess-Williams, R., Mills, K. A., Kang, S. H., Farr, S. E., Grant, G. D., Perkins, A., Davey, A., Anoopkumar-Dukie, S. (2013). Alterations in acetylcholine, PGE2 and IL6 release from urothelial cells following treatment with pyocyanin and lipopolysaccharide. Toxicology in Vitro, 27(6), 1693-1698.

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© Copyright, Elsevier Ltd, 2013

2013 HERDC Submission. FoR code: 110312

Abstract

The effects of pseudomonal virulence factor pyocyanin, and LPS from Pseudomonas aeruginosa and Escherichia coli on urothelial mediator release and cytokine production were examined. RT4 urothelial cells were treated with pyocyanin (1-100 μM) or LPS (1-100 ng/mL) for 24-h. Effects were measured in terms of changes in cell viability, basal and stretch-induced acetylcholine (Ach) and PGE2 release, and inflammatory cytokines (IL-6 and IL-12) production. Twenty-four hour pyocyanin (100 μM) treatment significantly decreased urothelial cell viability, while stretch-induced Ach release response was inhibited. E. coli LPS (100 ng/mL) produced a similar response with an additional significant increase in basal Ach release. All three virulence factors significantly increased urothelial PGE2 release; under basal release for pyocyanin (100 μM), stretch-induced release for pseudomonal LPS (≥ 10 ng/mL) and both basal and stimulated release for E. coli LPS (≥ 10 ng/mL). IL-6 and IL-12 were not detected in control samples, however 24h treatment with pyocyanin (100 μM) or LPS (100 ng/mL) resulted in IL-6 release from urothelial cells. The changes in urothelial Ach and PGE2, and release of inflammatory cytokine IL-6 induced by exposure to the bacterial virulence factors may play a role in the symptoms of pain and urinary urgency experienced with urinary tract infections.

Citation Information
Catherine M. McDermott, Russ Chess-Williams, Kylie A. Mills, Sung-Hung Kang, et al.. "Alterations in acetylcholine, PGE2 and IL6 release from urothelial cells following treatment with pyocyanin and lipopolysaccharide" oxicology in Vitro (2013) ISSN: 0887-2333
Available at: http://works.bepress.com/catherine_mcdermott/11/