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Assessment of a Metaviromic Dataset Generated from Nearshore Lake Michigan
Marine and Freshwater Research
  • Siobhan C Watkins, Loyola University Chicago
  • Neil Kuehnle, Loyola University Chicago
  • C Anthony Ruggeri, Loyola University Chicago
  • Kema Malki, Loyola University Chicago
  • Katherine Bruder, Loyola University Chicago
  • Jinan Elayyan, Loyola University Chicago
  • Kristina Damisch, Loyola University Chicago
  • Naushin Vahora, Loyola University Chicago
  • Paul O'Malley, Loyola University Chicago
  • Brianne Ruggles-Sage, Loyola University Chicago
  • Zachary Romer, Loyola University Chicago
  • Catherine Putonti, Loyola University Chicago
Document Type
Article
Publication Date
1-1-2015
Disciplines
Abstract
Bacteriophages are powerful ecosystem engineers. They drive bacterial mortality rates and genetic diversity, and affect microbially mediated biogeochemical processes on a global scale. This has been demonstrated in marine environments; however, phage communities have been less studied in freshwaters, despite representing a potentially more diverse environment. Lake Michigan is one of the largest bodies of freshwater on the planet, yet to date the diversity of its phages has yet to be examined. Here, we present a composite survey of viral ecology in the nearshore waters of Lake Michigan. Sequence analysis was performed using a web server previously used to analyse similar data. Our results revealed a diverse community of DNA phages, largely comprising the order Caudovirales. Within the scope of the current study, the Lake Michigan virome demonstrates a distinct community. Although several phages appeared to hold dominance, further examination highlighted the importance of interrogating metagenomic data at the genome level. We present our study as baseline information for further examination of the ecology of the lake. In the current study we discuss our results and highlight issues of data analysis which may be important for freshwater studies particularly, in light of the complexities associated with examining phage ecology generally
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Author Posting. © CSIRO Publishing, 2015. This article is posted here by permission of CSIRO for personal use, not for redistribution. The article was published in Marine and Freshwater Research, 2015, http://dx.doi.org/10.1071/MF15172

Creative Commons License
Creative Commons Attribution-Noncommercial-No Derivative Works 3.0
Citation Information
Watkins, SC et al. "Assessment of a metaviromic dataset generated from nearshore Lake Michigan." Marine and Freshwater Research, 2015.