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Synthesis and application of organomercury haptens for enzyme-linked immunoassay for inorganic and organic mercury
Analytical Biochemistry (2010)
  • Caryn K. Prudente, University of Southern Maine
  • Rebekah Sirois, University of Southern Maine
  • Shaun Cote, University of Southern Maine
Abstract
Two organomercury haptens were synthesized via the classical oxymercuration reaction. An intramolecular oxymercuration reaction was the strategy employed to prepare a structurally simple, but chemically robust, organomercury hapten that was conjugated to chicken immunoglobulin G (IgG). The resulting immunogen afforded mouse anti-mercury antibodies that were evaluated in an enzyme-linked immunosorbent assay (ELISA).
Keywords
  • Haptens,
  • Organomercury compounds,
  • Enzyme-linked immunosorbent assay
Disciplines
Publication Date
September, 2010
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Citation Information
Caryn K. Prudente, Rebekah Sirois and Shaun Cote. "Synthesis and application of organomercury haptens for enzyme-linked immunoassay for inorganic and organic mercury" Analytical Biochemistry Vol. 404 Iss. 2 (2010)
Available at: http://works.bepress.com/caryn_prudente/2/