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Article
Retinal patterning in the zebrafish mutant cyclops
Systems and Psychosocial Advances Research Center Publications and Presentations
  • Carl E. Fulwiler, University of Massachusetts Medical School
  • Ellen A. Schmitt, Harvard University
  • Jennie Min-Jung Kim, Harvard Medical School
  • John E. Dowling, Harvard University
UMMS Affiliation
Department of Psychiatry
Date
5-19-1997
Document Type
Article
Medical Subject Headings
Animals; Cell Differentiation; Cell Division; DNA; Embryo, Nonmammalian; Heterozygote; *Mutation; Neurons; Optic Nerve; Phenotype; Retina; Zebrafish
Abstract
Determination of cell fate in the vertebrate retina has been shown to be largely independent of lineage. After cell fates are determined, retinal neurons become organized in a precise laminar pattern. The mechanisms for this patterning could involve morphogens distributed in gradients or, alternatively, direct cell-cell interactions. In the zebrafish mutant cyclops (Cyc(b16)), most embryos have two partial retinas joined in the ventral midline. This presents developing retinal cells near the midline with abnormal cellular environments, whereas laterally the pattern of developing cells is normal. We examined the consequences of this for patterning in the mutant's retina. We found that the retinas are joined in the midline at the apical surfaces of the photoreceptor layers. A laminar pattern emerges in the midline that preserves normal positional relationships between retinal cell types locally but is abnormal with respect to patterning over the entire retina. Lateral to the midline, retinal patterning appears normal. Metabolic labeling experiments showed that late rounds of DNA synthesis precede the emergence of the novel pattern in this midline region. We conclude that these observations in the cyclops mutant are compatible with mechanisms of pattern formation in the retina involving local cell interactions.
Rights and Permissions
Citation: J Comp Neurol. 1997 May 19;381(4):449-60.
Related Resources
Link to Article in PubMed
PubMed ID
9136802
Citation Information
Carl E. Fulwiler, Ellen A. Schmitt, Jennie Min-Jung Kim and John E. Dowling. "Retinal patterning in the zebrafish mutant cyclops" Vol. 381 Iss. 4 (1997) ISSN: 0021-9967 (Linking)
Available at: http://works.bepress.com/carl_fulwiler/2/