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Article
Simple Adhesive-Tape-Based Sampling of Tomato Surfaces Combined with Rapid Fluorescence In Situ Hybridization for Salmonella Detection
Applied and Environmental Microbiology
  • Bledar Bisha, Iowa State University
  • Byron F. Brehm-Stecher, Iowa State University
Document Type
Article
Publication Date
3-1-2009
DOI
10.1128/AEM.01944-08
Abstract

A simple adhesive-tape-based method for sampling of tomato surfaces was combined with fluorescence in situ hybridization for rapid culture-independent detection of Salmonella strains. Tapes could also be placed face-down on selective agar for on-tape enrichment of captured Salmonella cells. Overlay of cell-charged tapes with small volumes of liquid enrichment media enabled subsequent detection of tape-captured Salmonella via flow cytometry.

Comments

This article is from Applied and Environmental Microbiology 75, no. 5 (March 2009): 1450–1455, doi:10.1128/AEM.01944-08.

Copyright Owner
American Society for Microbiology
Language
en
Date Available
January 16, 2013
File Format
application/pdf
Citation Information
Bledar Bisha and Byron F. Brehm-Stecher. "Simple Adhesive-Tape-Based Sampling of Tomato Surfaces Combined with Rapid Fluorescence In Situ Hybridization for Salmonella Detection" Applied and Environmental Microbiology Vol. 75 Iss. 5 (2009) p. 1450 - 1455
Available at: http://works.bepress.com/byron_brehmstecher/3/