Rapid Identification of Staphylococcus aureus and Methicillin Resistance by Flow Cytometry Using a Peptide Nucleic Acid ProbeJournal of Clinical Microbiology
AbstractA total of 56 Staphylococcus aureus isolates incubated for 2 h in the presence or absence of oxacillin were analyzed by flow cytometry after labeling with an S. aureus-specific peptide nucleic acid (PNA) probe. Two defined ratios, the paired signal count ratio (PSCR) and the gate signal count ratio (GSCR), differentiated methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) with sensitivities of 100% each and specificities of 96% and 100%, respectively.
Copyright OwnerAmerican Society for Microbiology
Citation InformationNabin K. Shrestha, Nikole M. Scalera, Deborah A. Wilson, Byron F. Brehm-Stecher, et al.. "Rapid Identification of Staphylococcus aureus and Methicillin Resistance by Flow Cytometry Using a Peptide Nucleic Acid Probe" Journal of Clinical Microbiology Vol. 49 Iss. 9 (2011) p. 3383 - 3385
Available at: http://works.bepress.com/byron_brehmstecher/2/