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Article
Rapid Identification of Staphylococcus aureus and Methicillin Resistance by Flow Cytometry Using a Peptide Nucleic Acid Probe
Journal of Clinical Microbiology
  • Nabin K. Shrestha, Cleveland Clinic
  • Nikole M. Scalera, Cleveland Clinic
  • Deborah A. Wilson, Cleveland Clinic
  • Byron F. Brehm-Stecher, Iowa State University
  • Gary W. Procop, Cleveland Clinic
Document Type
Article
Publication Date
9-1-2011
DOI
10.1128/JCM.01098-11
Abstract
A total of 56 Staphylococcus aureus isolates incubated for 2 h in the presence or absence of oxacillin were analyzed by flow cytometry after labeling with an S. aureus-specific peptide nucleic acid (PNA) probe. Two defined ratios, the paired signal count ratio (PSCR) and the gate signal count ratio (GSCR), differentiated methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) with sensitivities of 100% each and specificities of 96% and 100%, respectively.
Comments

This article is from Journal of Clinical Microbiology 49, no. 9 (September 2011): 3383–3385, doi:10.1128/JCM.01098-11.

Copyright Owner
American Society for Microbiology
Language
en
File Format
application/pdf
Citation Information
Nabin K. Shrestha, Nikole M. Scalera, Deborah A. Wilson, Byron F. Brehm-Stecher, et al.. "Rapid Identification of Staphylococcus aureus and Methicillin Resistance by Flow Cytometry Using a Peptide Nucleic Acid Probe" Journal of Clinical Microbiology Vol. 49 Iss. 9 (2011) p. 3383 - 3385
Available at: http://works.bepress.com/byron_brehmstecher/2/