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Article
Hepatocyte-specific gene expression from integrated lentiviral vectors
The Journal of Gene Medicine
  • Kathryn L Nash
  • Bushra Jamil, Aga Khan University
  • Alison J Maguire
  • Graeme J.M. Alexander
  • Andrew Lever, University of Cambridge
Publication Date
1-1-2004
Document Type
Article
Abstract
BACKGROUND: For many applications, efficient gene therapy will require long-term, organ-specific therapeutic gene expression. Lentiviral vectors based on HIV-1 are promising gene delivery vehicles due to their ability to integrate transgenes into non-dividing cells. Many experimental vectors express transgenes under the control of the cytomegalovirus (CMV) immediate-early gene promoter. Although this promoter directs strong gene expression in vitro, it may be shut off rapidly in vivo. This study explores the potential of HIV-1-based vectors to transduce hepatocytes and compares gene expression from different promoters in integrated vectors. METHODS: HIV-1-based vector plasmids expressing the green fluorescent protein (GFP) under the control of the CMV promoter, the alpha-1 antitrypsin gene promoter or promoters derived from the hepatitis B virus (HBV) genome were used to compare expression in transfected and transduced cell lines. RESULTS: Hepatocyte cell lines differed strikingly in their transfectability. Transduction with replication-deficient HIV-1-based vector particles incorporating the different promoter elements was uniformly effective in hepatocyte and non-hepatocyte lines. However, in hepatocytes, only the CMV, alpha-1 antitrypsin and HBV core but not HBV surface promoters were able to produce GFP expression. Addition of the HBV enhancer 2 element improved the transducing ability of the HBV surface promoter and suppressed expression in non-hepatocytes increasing specificity for hepatocytes.CONCLUSIONS: Integrated lentiviral vectors can be used to direct transgene expression in liver cells both promiscuously and specifically. Promoters derived from the alpha-1 antitrypsin gene or HBV are alternatives to the CMV promoter. Inclusion of the HBV enhancer 2 permits strong liver-specific gene expression in vitro
Citation Information
Kathryn L Nash, Bushra Jamil, Alison J Maguire, Graeme J.M. Alexander, et al.. "Hepatocyte-specific gene expression from integrated lentiviral vectors" The Journal of Gene Medicine Vol. 6 Iss. 9 (2004) p. 974 - 983
Available at: http://works.bepress.com/bushra_jamil/33/