"Imaging the Evolution of Reactivation Pulmonary Tuberculosis in Mice Using 18F-FDG PET" by Allison M. Murawski

Article

Imaging the Evolution of Reactivation Pulmonary Tuberculosis in Mice Using 18F-FDG PET

Journal of Nuclear Medicine (2014)

Allison M. Murawski, Johns Hopkins University
Saumya Gurbani, Johns Hopkins University
Jamie S. Harper, Johns Hopkins University
Mariah Klunk, Johns Hopkins University
Laurent Younes, Johns Hopkins University
Bruno M. Jedynak, Portland State University
Sanjay K. Jain, Johns Hopkins University

Abstract
Latent tuberculosis infection affects one third of the world’s population and can reactivate (relapse) decades later. However, current technologies, dependent on postmortem analyses, cannot follow the temporal evolution of disease.

Methods
C3HeB/FeJ mice, which develop necrotic and hypoxic tuberculosis lesions, were aerosol-infected with Mycobacterium tuberculosis. PET and CT were used to serially image the same cohort of infected mice through pretreatment, tuberculosis treatment, and subsequent development of relapse.

Results
A novel diffeomorphic registration was successfully used to monitor the spatial evolution of individual pulmonary lesions. Although most lesions during relapse developed in the same regions as those noted during pretreatment, several lesions also arose de novo within regions with no prior lesions.

Conclusion
This study presents a novel model that simulates infection and reactivation disease as seen in humans and could prove valuable to study tuberculosis pathogenesis and evaluate novel therapeutics.

Disciplines

Statistics and Probability

Publication Date

October, 2014

DOI

10.2967/jnumed.114.144634

Citation Information

Murawski, A. M., Gurbani, S., Harper, J. S., Klunk, M., Younes, L., Jain, S. K., & Jedynak, B. M. (2014). Imaging the evolution of reactivation pulmonary tuberculosis in mice using 18F-FDG PET. Journal Of Nuclear Medicine: Official Publication, Society Of Nuclear Medicine, 55(10), 1726-1729.