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At Least Four Distinct Circadian Regulatory Mechanisms Required for All Phases of Rhythms in mRNA Amount
Journal of Biological Rhythms
  • Sigrid Jacobshagen, Western Kentucky University
  • Bruce Kessler, Western Kentucky University
  • Claire Rinehart, Western Kentucky University
Publication Date

This research was supported by the National Institutes of Health and the National Center for Research Resources Grant P20 RR16481 as well as the National Institute of General Medical Sciences Grant 1R15GM076079-01A1.

The final, definitive version of this paper has been published in Journal of Biological Rhythms, v.23, no.6 (2008): 511-524 by SAGE Publications Ltd., SAGE Publications Inc. All rights reserved. c2008.


Since the advent of techniques to investigate gene expression on a large scale, numerous circadian rhythms in mRNA abundance have been reported. These rhythms generally differ in amplitude and phase. First studies on circadian rhythms of transcription on a large scale are also emerging. We investigated to what extent the same circadian regulatory mechanism of transcription can give rise to rhythms in RNA amount that differ in phase solely based on a parameter that is not regulated by the circadian clock. Using a discrete-time approach, we modeled a sinusoidal rhythm in transcription with various constant exponential RNA decay rates. We found that the slower the RNA is degraded the later the phase of the RNA amount rhythm compared to the phase of the transcriptional rhythm. However, we also found that the phase of the rhythm in RNA amount is limited to a time frame spanning the first quarter of the period following the phase of the transcriptional rhythm. This finding is independent of the amplitude and vertical shift of the transcriptional rhythm or even of the way RNA degradation is modeled. We confirmed our results with a continuous-time model, which also allowed us to derive a simple formula that relates the phase of a rhythm in mRNA amount solely to the phase and period of its sinusoidal transcriptional rhythm and its constant mRNA half-life. This simple formula even holds true for the best sinusoidal approximations of a non-sinusoidal rhythm of transcription and mRNA amount. When using our discrete-time approach to model constant rates of transcription with a sinusoidal RNA half-life, on the other hand, we found that varying the constant component of the system, i.e. the rate of transcription, does not change the phase of the rhythm in RNA amount. In summary, our data show that at least four distinct circadian regulatory mechanisms are required to allow for all phases in rhythms of RNA amount, one for each quarter of the period.

Citation Information
Sigrid Jacobshagen, Bruce Kessler and Claire Rinehart. "At Least Four Distinct Circadian Regulatory Mechanisms Required for All Phases of Rhythms in mRNA Amount" Journal of Biological Rhythms Vol. 23 Iss. 6 (2008) p. 511 - 524
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