Secondary structure formation precedes tertiary structure in the refolding of ribonuclease ABiochimica et Biophysica Acta (1992)
AbstractThe kinetics of refolding of ribonuclease A were monitored by circular dichroism (CD), tyrosine fluorescence and absorbance in the −40 to −10°C range using a methanol cryosolvent. The native-like far-ultraviolet CD signal returned in te dead-tinme of the mixing, whereas the native absorbance and fluorescence signals returned in a multiphasic process at rates several orders of magnitude more slowly. Thus the secondary structure was formed much more rapidly than the tertiary structure. In addition, the absorbance signal showed evidence of an early intermediate in which one, or more, tyrosine residues was in a transiently more polar environment. A total of four kinetic phases were observed by absorbance in refolding, the slowest two of which had energies of activation consistent with proline isomerization. A refolding scheme involving initial hydrophobic collapse, concurrent with secondary structure formation, followed by much slower rearrangement to the native tertiary structure is proposed.
- ribonuclease A,
- tertiary structure
Citation InformationBrooke Lustig and A L Fink. "Secondary structure formation precedes tertiary structure in the refolding of ribonuclease A" Biochimica et Biophysica Acta Vol. 1121 (1992)
Available at: http://works.bepress.com/brooke_lustig/9/