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Article
Consistencies of individual DNA base-amino acid interactions in structures and sequences
Nucleic Acids Research (1995)
  • Brooke Lustig , San Jose State University
  • R L Jernigan
Abstract
Amino acid-amino acid interaction energies have been derived from crystal structure data for a number of years. Here is reported the first derivation of normalized relative interaction from binding data for each of the four bases interacting with a specific amino acid, utilizing data from combinatorial multiplex ONA binding of zinc finger domains [Desjarlais, J. R. and Berg, J. M. (1994) Proc. Natl. Acad. Sci. USA, 91, 11099–11103]. The five strongest Interactions are observed for lysine-guanine, lysine-thymine, arginine- guanine, aspartic acid-cytosine and asparagineadenine. These rankings for interactions with the four bases appear to be related to base-amino acid partial charges. Also, similar normalized relative interaction energies are derived by using DNA binding data for Cro and X repressors and the R2R3 c-Myb protein domain [Takeda, Y., Sarai, A. and Rivera, V. M. (1989) Proc. Natl. Acad. Sci. USA, 86, 439–443; Sarai, A. and Takeda, Y. (1989) Proc. Natl. Acad. Sci. USA, 86, 6513–6517; Ogata, K. et al. (1995) submitted]. These energies correlate well with the combinatorial multiplex energies, and the strongest cases are similar between the two sets. They also correlate well with similar relative interaction energies derived directly from frequencies of bases in the bacteriophage X operator sequences. These results suggest that such potentials are general and that extensive combinatorial binding studies can be used to derive potential energies for DNA-protein interactions.
Keywords
  • individual DNA base-amino acid
Publication Date
1995
Publisher Statement
Copyright © 1995 Oxford University Press.The article was originally published in Nucleic Acids Research and can be found online at: http://dx.doi.org/10.1080/07391102.1994.10508093.
Citation Information
Brooke Lustig and R L Jernigan. "Consistencies of individual DNA base-amino acid interactions in structures and sequences" Nucleic Acids Research Vol. 23 Iss. 22 (1995)
Available at: http://works.bepress.com/brooke_lustig/6/