Skip to main content
Article
Impact of apoE deficiency during synaptic remodeling in the mouse olfactory bulb
Neuroscience Letters (2008)
  • Ikemefuna Nwosu, Eastern Illinois University
  • Salina Gairhe, Eastern Illinois University
  • Robert G. Struble, Southern Illinois University Carbondale
  • Britto P. Nathan, Eastern Illinois University
Abstract

In this study we examined the role of apoE on the rate of synaptic recovery in the olfactory bulb (OB) following olfactory epithelium (OE) lesioning in mice. We used both immunoblotting and immunohistochemical techniques to compare the density of OB synaptophysin (Syn, a synaptic marker) in apoE-gene deficient/knockout (KO) mice and wild-type (WT) mice following OE lesion. We found that the whole bulb concentrations of Syn, measured by immunoblotting, declined sharply following injury in both WT and KO mice during the degenerative phase (3–7 days). After this initial decline, the Syn concentration gradually increased to normal levels by 56 days in WT mice. In contrast, Syn concentration in KO mice did not recover by day 56 when Syn density in WT was essentially normal. Glomerular Syn density, measured by immunohistochemistry, found a lower density in KO mice at all time points post lesion. This lower concentration of whole bulb Syn parallels the slower recovery of glomerular area in KO mice. The data indicate that apoE deficiency in KO mice is associated with a delayed recovery of the glomerular area and a slower recovery in Syn concentration in the OB.

Keywords
  • apoE,
  • synaptophysin,
  • olfactory bulb,
  • glia,
  • olfactory nerve,
  • glial proteins,
  • transgenic mice
Publication Date
August 29, 2008
Publisher Statement
Published in final edited form as: Neurosci Lett. 2008 August 29; 441(3): 282–285. doi:10.1016/j.neulet.2008.05.117
Citation Information
Ikemefuna Nwosu, Salina Gairhe, Robert G. Struble and Britto P. Nathan. "Impact of apoE deficiency during synaptic remodeling in the mouse olfactory bulb" Neuroscience Letters Vol. 441 Iss. 3 (2008)
Available at: http://works.bepress.com/britto_nathan/5/