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Article
Rat Parotid Gland Amylase: Evidence for Alterations in an Exocrine Protein with Increased Age
Mechanisms of Ageing and Development
  • B. J. Baum
  • R. L. Levine
  • Brian L. Kuyatt, Cedarville University
  • D. B. Sogin
Document Type
Article
Publication Date
5-1-1982
DOI
http://dx.doi.org/10.1016/0047-6374(82)90047-1
Abstract
The content of alpha-amylase, the major exocrine secretory protein from rat parotid glands, was studied in young adult and aged rat tissue. alpha-Amylase protein was determined with an enzyme-linked immunosorbent assay. This employed antisera, produced against alpha-amylase purified from young adult rats, but which recognized and precipitated alpha-amylase enzyme activity equally well from both age groups. The parotid gland content of alpha-amylase was reduced about 50% in aged rats. Furthermore, the percentage of total gland protein which was alpha-amylase was decreased about 40% in aged animals. The data suggest that a somewhat specific alteration in alpha-amylase production (synthesis and/or degradation) occurs in parotid glands from aged rats. In addition, alpha-amylase functional activity was followed. The specific enzyme activity (U amylase activity per mg immunoreactive amylase) was about 35% higher in extracts from aged rat parotid glands compared to that of young adult glands.
Keywords
  • Aging,
  • amylases,
  • parotid gland,
  • proteins,
  • rats
PubMed ID
6180260
Citation Information
B. J. Baum, R. L. Levine, Brian L. Kuyatt and D. B. Sogin. "Rat Parotid Gland Amylase: Evidence for Alterations in an Exocrine Protein with Increased Age" Mechanisms of Ageing and Development Vol. 19 Iss. 1 (1982) p. 27 - 35 ISSN: 0047-6374
Available at: http://works.bepress.com/brian_kuyatt/9/