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Article
Quantitation of Smooth Muscle Proliferation in Cultured Aorta: A Color Image Analysis Method for the Macintosh
Analytical and Quantitative Cytology and Histology
  • Brian L. Kuyatt, Cedarville University
  • C. A. Reidy
  • K. Y. Hui
  • W. H. Jordan
Document Type
Article
Publication Date
4-1-1993
Abstract
Color image analysis was used to assess proliferative changes in smooth muscle cells from cultured segments of rabbit aortas. Proliferating cells were labeled with bromodeoxyuridine (BrdU) and visualized by immunohistochemical staining of histologic sections. A Macintosh IIfx computer with a Data Translation digitizer board, Javelin color camera and a color-enhanced version of National Institutes of Health Image 1.31 image analysis software (ColorImage 1.31) was used to acquire red, green and blue (RGB)-filtered grayscale images from microscopic slides of control and treated aortas. The BrdU-labeled (brown) and nonlabeled, hematoxylin (blue)-stained nuclei were identified on the RGB gray-scale images using a thresholding technique and sampled for nuclear number and area. An increase in the number of BrdU-labeled nuclei in the region of experimental perturbation was demonstrated by this semiautomated method. Thus, this Macintosh-based color image analysis method proved to be effective in rapidly quantitating immunohistochemically defined smooth muscle proliferation in microscopic tissues.
Keywords
  • Color image analysis,
  • smooth muscle cells,
  • aorta,
  • rabbits,
  • computer assisted image interpretation,
  • immunohistochemistry
PubMed ID
8318131
Citation Information
Brian L. Kuyatt, C. A. Reidy, K. Y. Hui and W. H. Jordan. "Quantitation of Smooth Muscle Proliferation in Cultured Aorta: A Color Image Analysis Method for the Macintosh" Analytical and Quantitative Cytology and Histology Vol. 15 Iss. 2 (1993) p. 83 - 87 ISSN: 0884-6812
Available at: http://works.bepress.com/brian_kuyatt/20/