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Effect of Photobiomodulation on Vinblastine-Poisoned Murine HERS Cells
Photomedicine and Laser Surgery
  • Brian D. Hodgson, Marquette University
  • B. Pyszka
  • Michele M Henry, Medical College of Wisconsin
  • E. Buchmann, Medical College of Wisconsin
  • Harry T. Whelan
Document Type
Format of Original
5 p.
Publication Date
Mary Ann Liebert Inc.
Original Item ID
doi: 10.1089/pho.2010.2794

Objective: The aim of this study was to investigate the effect of near-infrared (NIR) photobiomodulation on the proliferation and glutathione levels in murine Hertwig's epithelial root sheath (HERS) cells after poisoning with vinblastine. Background: Photobiomodulation has been shown to improve wound healing in a number of animal models. There have been no studies on the effect of photobiomodulation on cancer-related chemotherapy injury to the cells that initiate tooth root growth. Materials and Methods: Control groups consisted of murine HERS cells without vinblastine (VB−) and cells with vinblastine at 10, 20, and 30 ng/mL (VB10, VB20, and VB30). Experimental groups consisted of these same groups with light therapy (VB-L, VB10L, VB20L, and VB30L). The cells were exposed to vinblastine for 1 h. Photobiomodulation consisted of a 75-cm2 gallium-aluminum-arsenide light-emitting diode (LED) array at an energy density of 12.8 J/cm2, delivered with 50 mW/cm2 power over 256 s. Results: Vinblastine alone significantly decreased HERS cell proliferation and glutathione levels at all concentrations (VB10 [−55%, p < 1.0 × 10−8]; VB20 [−72%, p < 1.0 × 10−9]; VB30 [−80%, p < 1.0 × 10−10]; and VB10 [−36%, p < 0.0001]; VB20 [−49%, p < 1.0 × 10−6]; VB30 [−53%, p < 1.0 × 10−7] respectively). Photobiomodulation significantly increased cell proliferation at all levels of vinblastine exposure (VB10L [+50%, p < 0.0001]; VB20L [+45%, p < 0.05]; VB30 [+39%, p < 0.05]) but not of the control (+22%, p  = 0.063). The photobiomodulation significantly increased glutathione production in all concentrations of vinblastine except 20 ng/mL (VB10L [+39%, p = 0.007]; VB20L [+19%, p = 0.087]; VB30 [+14%, p = 0.025]) and the control (+12%, p = 0.13). Conclusions: Photobiomodulation demonstrated an improvement in proliferation and glutathione levels in vinblastine-poisoned murine HERS cells.


Published version. Photomedicine and Laser Surgery, Volume 29, No. 4 (2011): 233–237. DOI. © 2011 Mary Ann Liebert Inc. Used with permission.

Citation Information
Brian D. Hodgson, B. Pyszka, Michele M Henry, E. Buchmann, et al.. "Effect of Photobiomodulation on Vinblastine-Poisoned Murine HERS Cells" Photomedicine and Laser Surgery (2010) ISSN: 1549-5419
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