Skip to main content
Article
Age Alterations in Extent and Severity of Experimental Intranasal Infection with Chlamydophila pneumoniae in BALB/c Mice
Infection and Immunity
  • Christopher Scott Little, Philadelphia College of Osteopathic Medicine
  • Andrew Bowe, Philadelphia College of Osteopathic Medicine
  • Richard Lin
  • Jason Litsky
  • Robert M Fogel, Philadelphia College of Osteopathic Medicine
  • Brian J. Balin, PhD, Philadelphia College of Osteopathic Medicine
  • Kerin L. Fresa-Dillon, Philadelphia College of Osteopathic Medicine
Document Type
Article
Publication Date
3-1-2005
Abstract
The intracellular bacterium Chlamydophila ("Chlamydia") pneumoniae is a pathogen for several respiratory diseases and may be a factor in the pathogenesis of chronic diseases of aging including atherosclerosis and Alzheimer's disease. We assessed whether aging is coupled with increased burden of infection in BALB/c mice after intranasal infection by C. pneumoniae. Six- and twenty-month-old BALB/c mice were infected intranasally with 5 x 10(4) inclusion forming units (IFU) or 5 x 10(5) IFU of C. pneumoniae. Lung, brain, and heart tissue were analyzed for infectious C. pneumoniae and for Chlamydophila antigen by immunohistochemistry. At both doses, aging was associated with a decreased proportion of animals that cleared infection from the lung and greater burden of infectious organism within the lung. We observed dose-dependent spread to the heart/ascending aorta in animals infected with C. pneumoniae. In mice given 5 x 10(4) IFU, spread to the heart by day 14 was only observed in old mice. By day 28, all animals inoculated with 5 x 10(4) IFU showed evidence of spread to the heart, although higher C. pneumoniae titers were observed in the hearts from old mice. In mice inoculated with 5 x 10(5) IFU, spread of C. pneumoniae to the heart was evident by day 14, with no discernible age effect. C. pneumoniae was also recovered from the central nervous system (brain and olfactory bulb) of all mice by day 28 postinfection, with higher C. pneumoniae titers in old animals than in young animals. Our results suggest that infection with C. pneumoniae may be more severe in old animals.
PubMed ID
15731073
Comments

This article was published in Infection and Immunity, Volume 73, Issue 3, March 2005, Pages 1723-34.

The published version is available at http://dx.doi.org/10.1128/IAI.73.3.1723-1734.2005

Copyright © 2005 by the American Society for Microbiology

Citation Information
Christopher Scott Little, Andrew Bowe, Richard Lin, Jason Litsky, et al.. "Age Alterations in Extent and Severity of Experimental Intranasal Infection with Chlamydophila pneumoniae in BALB/c Mice" Infection and Immunity Vol. 73 Iss. 3 (2005) p. 1723 - 1734
Available at: http://works.bepress.com/brian_balin/42/