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Article
Determination of Dimethyl Trisulfide in Rabbit Blood Using Stir Bar Sorptive Extraction Gas Chromatography-mass Spectrometry
Journal of Chromatography A
  • Erica Mananadhar, South Dakota State University
  • Nujud Maslamani, South Dakota State University
  • Ilona Petrikovics, Sam Houston State University
  • Gary A. Rockwood, US Army Medical Research Institute of Chemical Defense
  • Brian A. Logue, South Dakota State University
Document Type
Article
Publication Date
8-1-2016
Abstract

Cyanide poisoning by accidental or intentional exposure poses a severe health risk. The current Food and Drug Administration approved antidotes for cyanide poisoning can be effective, but each suffers from specific major limitations concerning large effective dosage, delayed onset of action, or dependence on enzymes generally confined to specific organs. Dimethyl trisulfide (DMTS), a sulfur donor that detoxifies cyanide by converting it into thiocyanate (a relatively nontoxic cyanide metabolite), is a promising next generation cyanide antidote. Although a validated analytical method to analyze DMTS from any matrix is not currently available, one will be vital for the approval of DMTS as a therapeutic agent against cyanide poisoning. Hence, a stir bar sorptive extraction (SBSE) gas chromatography – mass spectrometry (GC–MS) method was developed and validated for the analysis of DMTS from rabbit whole blood. Following acid denaturation of blood, DMTS was extracted into a polydimethylsiloxane-coated stir bar. The DMTS was then thermally desorbed from the stir bar and analyzed by GC–MS. The limit of detection of DMTS using this method was 0.06 μM with dynamic range from 0.5–100 μM. For quality control standards, the precision, as measured by percent relative standard deviation, was below 10%, and the accuracy was within 15% of the nominal concentration. The method described here will allow further investigations of DMTS as a promising antidote for cyanide poisoning.

Format
application/pdf
DOI of Published Version
10.1016/j.chroma.2016.07.046
Publisher
Elsevier
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S.
Citation Information
Erica Mananadhar, Nujud Maslamani, Ilona Petrikovics, Gary A. Rockwood, et al.. "Determination of Dimethyl Trisulfide in Rabbit Blood Using Stir Bar Sorptive Extraction Gas Chromatography-mass Spectrometry" Journal of Chromatography A Vol. 1461 (2016) p. 10 - 17
Available at: http://works.bepress.com/brian-logue/11/