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Article
Prevention of granuloma development in the mouse by using T cell hybridoma products.
Journal of Immunology (1984)
  • C. H. Ginsburg
  • J. T. Drambrauskas
  • R. B. Whitaker, University of Southern Maine
  • Z. M. Falchuk
  • M. I. Greene
Abstract

The ability of an azobenzenearsonate (ABA)-specific suppressor T cell factor, a soluble extract from first order suppressor T cells (Ts1), and suppressor molecules produced by a long-term T cell hybridoma to regulate ABA-specific granuloma formation was studied. ABA-derivatized syngeneic spleen cells (ABA-SC) administered subcutaneously induced persistent delayed-type hypersensitivity (DTH) responses, detected by footpad swelling and hapten-specific granuloma formation by 72 and 96 hr after challenge with ABA-bovine serum albumin coupled to polyacrylamide beads (ABA-BSA-PAB). Soluble factors from ABA-specific Ts1 prevented DTH and granulomatous development after subcutaneous administration of ABA-SC. Moreover, the in vivo administration of a factor that is derived from a Ts1 functioning hybrid cell line induced a second set of suppressor cells (Ts2) that upon transfer to syngeneic ABA-primed mice were able to inhibit granuloma formation in the footpad, as well as in the gastrointestinal tract after challenge with ABA-BSA-PAB. These experiments demonstrate the dependence of the granulomatous reaction on T cell-mediated events, as well as the potential therapeutic efficacy of an antigen-specific suppressor T cell factor and a hybridoma T cell product in limiting antigen-specific granuloma formation in vivo.

Keywords
  • Granuloma
Publication Date
1984
Citation Information
C. H. Ginsburg, J. T. Drambrauskas, R. B. Whitaker, Z. M. Falchuk, et al.. "Prevention of granuloma development in the mouse by using T cell hybridoma products." Journal of Immunology Vol. 132 (1984)
Available at: http://works.bepress.com/blake_whitaker/4/