Article
Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination
J Biomed Opt
(2017)
Abstract
Confocal microscopy is an emerging technology for rapid imaging of freshly excised tissue without the need for frozen- or fixed-section processing. Initial studies have described imaging of breast tissue using fluorescence confocal microscopy with small regions of interest, typically 750 × 750 ?? ? m 2 . We present exploration with a microscope, termed confocal strip-mosaicking microscope (CSM microscope), which images an area of 2 × 2 ?? cm 2 of tissue with cellular-level resolution in 10 min of excision. Using the CSM microscope, we imaged 34 fresh, human, large breast tissue specimens from 18 patients, blindly analyzed by a board-certified pathologist and subsequently correlated with the corresponding standard fixed histopathology. Invasive tumors and benign tissue were clearly identified in CSM strip-mosaic images. Thirty specimens were concordant for image-to-histopathology correlation while four were discordant.
Disciplines
Publication Date
March, 2017
DOI
https://doi.org/10.1117/1.jbo.22.3.034002
Citation Information
Sanjee Abeytunge, Bjorg Larson, Gary Peterson, Monica Morrow, et al.. "Evaluation of breast tissue with confocal strip-mosaicking microscopy: a test approach emulating pathology-like examination" J Biomed Opt Vol. 22 Iss. 3 (2017) Available at: http://works.bepress.com/bjorg-larson/1/