We demonstrate that individual electron-hole pairs are resolved in a 1 cm2 by 4 mm thick silicon crystal (0.93 g) operated at ∼35 mK. One side of the detector is patterned with two quasiparticle-trap-assisted electro-thermal-feedback transition edge sensor arrays held near ground potential. The other side contains a bias grid with 20% coverage. Bias potentials up to ±160 V were used in the work reported here. A fiber optic provides 650 nm (1.9 eV) photons that each produce an electron-hole (e– h+) pair in the crystal near the grid. The energy of the drifting charges is measured with a phonon sensor noise σ ∼0.09 e– h+ pair. The observed charge quantization is nearly identical for h+s or e–s transported across the crystal.