Article
Disruption of Arabidopsis CHY1 Reveals an Important Role of Metabolic Status in Plant Cold Stress Signaling
Molecular Plant
(2009)
Abstract
To study cold signaling, we screened for Arabidopsis mutants with altered cold-induced transcription of a firefly luciferase reporter gene driven by the CBF3 promoter (CBF3-LUC). One mutant, chy1-10, displayed reduced cold-induction of CBF3-LUC luminescence. RNA gel blot analysis revealed that expression of endogenous CBFs also was reduced in the chy1 mutant. chy1-10 mutant plants are more sensitive to freezing treatment than wild-type after cold acclimation. Both the wild-type and chy1 mutant plants are sensitive to darkness-induced starvation at warm temperatures, although chy1 plants are slightly more sensitive. This dark-sensitivity is suppressed by cold temperature in the wildtype but not in chy1. Constitutive CBF3 expression partially rescues the sensitivity of chy1-10 plants to dark treatment in the cold. The chy1 mutant accumulates higher levels of reactive oxygen species, and application of hydrogen peroxide can reduce cold-induction of CBF3-LUC in wild-type. Map-based cloning of the gene defective in the mutant revealed a nonsense mutation in CHY1, which encodes a peroxisomal b-hydroxyisobutyryl (HIBYL)–CoA hydrolase needed for valine catabolism and fatty acid b-oxidation. Our results suggest a role for peroxisomal metabolism in cold stress signaling, and plant tolerance to cold stress and darkness-induced starvation.
Keywords
- Cold stress,
- signal transduction,
- gene regulation,
- CHY1,
- b-hydroxyisobutyryl–CoA hydrolase
Disciplines
- Genetics,
- Biochemistry and
- Biology
Publication Date
January 1, 2009
DOI
10.1093/mp/ssn063
Citation Information
Chun-Hai Dong, Bethany K. Zolman, Bonnie Bartel, Byeong-ha Lee, et al.. "Disruption of Arabidopsis CHY1 Reveals an Important Role of Metabolic Status in Plant Cold Stress Signaling" Molecular Plant Vol. 2 Iss. 1 (2009) p. 59 - 72 Available at: http://works.bepress.com/bethany-zolman/1/