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Article
Lack of telomere shortening with age in mouse resting zone chondrocytes
Pediatric Publications and Presentations
  • Benjamin U. Nwosu, University of Massachusetts Medical School
  • Ola Nilsson, National Institutes of Health
  • Robert D. Mitchum, Jr., National Institutes of Health
  • Marilena Coco, National Institutes of Health
  • Kevin M. Barnes, National Institutes of Health
  • Jeffrey Baron, National Institutes of Health
UMMS Affiliation
Department of Pediatrics, Division of Endocrinology
Date
3-24-2005
Document Type
Article
Medical Subject Headings
Aging; Animals; Blotting, Southern; Cell Division; Chondrocytes; Growth Plate; Mice; Telomere
Disciplines
Abstract
BACKGROUND AND AIM: Telomeres are hexameric repeat sequences that flank eukaryotic chromosomes. The telomere hypothesis of cellular aging proposes that replication of normal somatic cells leads to progressive telomere shortening which induces replicative senescence. Previous studies suggest that growth plate chondrocytes have a finite proliferative capacity in vivo. We therefore hypothesized that telomere shortening in resting zone chondrocytes leads to replicative senescence. METHOD: To test this hypothesis we compared the telomere restriction fragment (TRF) length of Mus casteneus at 1, 4, 8, and 56 weeks of age. RESULTS AND CONCLUSIONS: We found that TRF length did not diminish measurably with age, suggesting that telomere shortening in resting zone chondrocytes is not the mechanism that limits proliferation of growth plate chondrocytes in vivo.
Rights and Permissions
Citation: Horm Res. 2005;63(3):125-8. Epub 2005 Mar 24. Link to article on publisher's site
Related Resources
Link to Article in PubMed
PubMed ID
15795509
Citation Information
Benjamin U. Nwosu, Ola Nilsson, Robert D. Mitchum, Marilena Coco, et al.. "Lack of telomere shortening with age in mouse resting zone chondrocytes" Vol. 63 Iss. 3 (2005) ISSN: 0301-0163 (Linking)
Available at: http://works.bepress.com/benjamin_nwosu/3/