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Article
Shaped 3D microcarriers for adherent cell culture and analysis
Microsystems & Nanoengineering
  • Chueh-Yu Wu, University of California, Los Angeles
  • Daniel Stoecklein, Iowa State University
  • Aditya Kommajosula, Iowa State University
  • Jonathan Lin, University of California, Los Angeles
  • Keegan Owsley, University of California, Los Angeles
  • Baskar Ganapathysubramanian, Iowa State University
  • Dino Di Carlo, University of California, Los Angeles
Document Type
Article
Publication Version
Published Version
Publication Date
8-13-2018
DOI
10.1038/s41378-018-0020-7
Abstract

Standard tissue culture of adherent cells is known to poorly replicate physiology and often entails suspending cells in solution for analysis and sorting, which modulates protein expression and eliminates intercellular connections. To allow adherent culture and processing in flow, we present 3D-shaped hydrogel cell microcarriers, which are designed with a recessed nook in a first dimension to provide a tunable shear-stress shelter for cell growth, and a dumbbell shape in an orthogonal direction to allow for self-alignment in a confined flow, important for processing in flow and imaging flow cytometry. We designed a method to rapidly design, using the genetic algorithm, and manufacture the microcarriers at scale using a transient liquid molding optofluidic approach. The ability to precisely engineer the microcarriers solves fundamental challenges with shear-stress-induced cell damage during liquid-handling, and is poised to enable adherent cell culture, in-flow analysis, and sorting in a single format.

Comments

This article is published as Wu, Chueh-Yu, Daniel Stoecklein, Aditya Kommajosula, Jonathan Lin, Keegan Owsley, Baskar Ganapathysubramanian, and Dino Di Carlo. "Shaped 3D microcarriers for adherent cell culture and analysis." Microsystems & Nanoengineering 4, no. 1 (2018): 21. DOI: 10.1038/s41378-018-0020-7. Posted with permission.

Creative Commons License
Creative Commons Attribution 4.0
Copyright Owner
The Authors
Language
en
File Format
application/pdf
Citation Information
Chueh-Yu Wu, Daniel Stoecklein, Aditya Kommajosula, Jonathan Lin, et al.. "Shaped 3D microcarriers for adherent cell culture and analysis" Microsystems & Nanoengineering Vol. 4 Iss. 1 (2018) p. 21
Available at: http://works.bepress.com/baskar-ganapathysubramanian/64/