SelectedWorks - Basil Nikolau

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Selected Works of Basil Nikolau

Professor of Biochemistry, Biophysics and Molecular Biology

Dr. Nikolau’s research interests focus on the biochemistry and molecular biology of biotin and biotin-containing enzymes and the regulation of plant lipid metabolism.

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About Basil Nikolau

Dr. Nikolau’s research interests focus on the biochemistry and molecular biology of biotin and biotin-containing enzymes and the regulation of plant lipid metabolism.

Present	Professor, Iowa State University ‐ Department of Biochemistry, Biophysics and Molecular Biology
Institution Organization Position Type Position Title Time Period to Options Current Position Affiliate my profile with this Institution

Biochemistry, Biophysics, and Structural Biology and Microbiology

3254 Molecular Bio

2437 Pammel Dr

Ames, IA 50011-1079

Phone: 515-294-9423

Fax: 515-294-0453

Email:

Peer-Reviewed Articles (63)

Kinetic, Structural, and Mutational Analysis of Acyl-CoA Carboxylase From Thermobifida ...

Frontiers in Molecular Biosciences (2021)

Kiran-Kumar Shivaiah, Bryon Upton and Basil J. Nikolau

Acyl-CoA carboxylases (AcCCase) are biotin-dependent enzymes that are capable of carboxylating more than one short chain acyl-CoA substrate. We have ...

Maize Glossy2 and Glossy2-like Genes Have Overlapping and Distinct Functions ...

Plant Physiology (2020)

Liza Esther Alexander, Yozo Okazaki, Michael A. Schelling, Aeriel Davis, et al.

Plant epidermal cells express unique molecular machinery that juxtapose the assembly of intracellular lipid components and the unique extracellular cuticular ...

Dual-Localized Enzymatic Components Constitute the Fatty Acid Synthase Systems in ...

Plant Physiology (2020)

Xin Guan, Yozo Okazaki, Rwisdom Zhang, Kazuki Saito, et al.

Plant fatty acid biosynthesis occurs in both plastids and mitochondria. Here, we report the identification and characterization of Arabidopsis (Arabidopsis ...

Mitochondrial Fatty Acid Synthase Utilizes Multiple Acyl Carrier Protein Isoforms

Plant Physiology (2020)

Xinyu Fu, Xin Guan, Rachel Garlock and Basil J. Nikolau

Acyl carrier protein (ACP) is a highly conserved cofactor protein that is required by Type II fatty acid synthases (FASs). ...

Metabolomic Profiling of Nicotiana Spp. Nectars Indicate That Pollinator Feeding ...

Metabolites (2020)

Fredy A. Silva, Elizabeth C. Chatt, Siti-Nabilla Mahalim, Adel Guirgis, et al.

Floral nectar is a rich secretion produced by the nectary gland and is offered as reward to attract pollinators leading ...

Failure to Maintain Acetate Homeostasis by Acetate-Activating Enzymes Impacts Plant ...

Plant Physiology (2020)

Xinyu Fu, Hannah Yang, Febriana Pangestu and Basil J. Nikolau

The metabolic intermediate acetyl-CoA links anabolic and catabolic processes and coordinates metabolism with cellular signaling by influencing protein acetylation. In ...

Evaluation of the functional role of the maize Glossy2 and ...

Liza Esther Alexander, Yozo Okazaki, Michael A. Schelling, Aeriel Davis, et al.

Plant epidermal cells express unique molecular machinery that juxtapose the assembly of intracellular lipid components and the unique extracellular cuticular ...

Non-Catalytic Subunits Facilitate Quaternary Organization of Plastidic Acetyl-CoA Carboxylase

Plant Physiology (2020)

Kiran-Kumar Shivaiah, Geng Ding, Bryon Upton and Basil J. Nikolau

Arabidopsis (Arabidopsis thaliana), like most dicotyledonous plants, expresses a multicomponent, heteromeric acetyl-CoA carboxylase (htACCase), which catalyzes the generation of the ...

Systems analyses of key metabolic modules of floral and extrafloral ...

Plant Physiology (2019)

Elizabeth C. Chatt, Siti-Nabilla Mahalim, Nur-Aziatull Mohd-Fadzil, Rahul Roy, et al.

Nectar is a primary reward mediating plant-animal mutualisms to improve plant fitness and reproductive success. In Gossypium hirsutum (cotton), four ...

An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo ...

Plant Direct (2019)

Erik M. Solhaug, Rahul Roy, Elizabeth C. Chatt, Peter M. Klinkenberg, et al.

Nectar is the main reward that flowers offer to pollinators to entice repeated visitation. Cucurbita pepo (squash) is an excellent ...

A single-cell platform for reconstituting and characterizing fatty acid elongase ...

PLoS One (2019)

Alexis A. Campbell, Kenna E. Stenback, Kayla Flyckt, Trang Hoang, et al.

Fatty acids of more than 18-carbons, generally known as very long chain fatty acids (VLCFAs) are essential for eukaryotic cell ...

Altering the Substrate Specificity of Acetyl-CoA Synthetase by Rational Mutagenesis ...

ACS Synthetic Biology (2019)

Naazneen Sofeo, Jason H. Hart, Brandon Butler, David J. Oliver, et al.

Acetyl-CoA synthetase (ACS) is a member of a large superfamily of enzymes that display diverse substrate specificities, with a common ...

Genetic and environmental variation impact the cuticular hydrocarbon metabolome on ...

BMC Plant Biology (2019)

Tesia Dennison, Wenmin Qin, Derek M. Loneman, Samson G. F. Condon, et al.

Background: Simple non-isoprenoid hydrocarbons accumulate in discrete regions of the biosphere, including within bacteria and algae as a carbon and/or ...

Identification of Active Site Residues Implies a Two-step Catalytic Mechanism ...

Biochemical Journal (2018)

Fuyuan Jing, Marna D. Yandeau-Nelson and Basil J. Nikolau

In plants and bacteria that use a Type II fatty acid synthase (FAS), isozymes of acyl-acyl carrier protein (ACP) thioesterase ...

Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon ...

Ames Laboratory Accepted Manuscripts (2018)

Patrick A. McVey, Liza E. Alexander, Xinyu Fu, Bo Xie, et al.

The visualization of foliage color in plants provides immediate insight into some of the compounds that exist in the leaf. ...

Sex-Dependent Variation of Pumpkin (Cucurbita maxima cv. Big Max) Nectar ...

Frontiers in Plant Science (2018)

Elizabeth C. Chatt, Patrick von Aderkas, Clay J. Carter, Derek Smith, et al.

Nectar is a floral reward that sustains mutualisms with pollinators, which in turn, improves fruit set. While it is known ...

Characterizing virus-induced gene silencing at the cellular level with in ...

Plant Methods (2018)

Sadie J. Burkhow, Nicole M. Stephens, Yu Mei, Maria Emilia Duenas, et al.

Background: Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few ...

Two distinct domains contribute to the substrate acyl chain length ...

Nature Communications (2018)

Fuyuan Jing, Le Zhao, Marna D. Yandeau-Nelson and Basil J. Nikolau

The substrate specificity of acyl-ACP thioesterase (TE) plays an essential role in controlling the fatty acid profile produced by type ...

Spatial Mapping and Profiling of Metabolite Distributions During Germination

Plant Physiology (2017)

Adam D. Feenstra, Liza E. Alexander, Zhihong Song, Andrew R. Korte, et al.

Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this ...

A robust and efficient method for the extraction of plant ...

PloS ONE (2017)

Derek M. Loneman, Layton Peddicord, Amani Al-Rashid, Basil J. Nikolau, et al.

Aerial plant organs possess a diverse array of extracellular surface lipids, including both non-polar and amphipathic constituents that collectively provide ...

High spatial resolution mass spectrometry imaging reveals the genetically programmed ...

The Plant Journal (2017)

Maria Emilia Duenas, Adam T. Klein, Liza E. Alexander, Marna D. Yandeau-Nelson, et al.

Metabolism in plants is compartmentalized among different tissues, cells and subcellular organelles. Mass spectrometry imaging (MSI) with matrix-assisted laser desorption ...

The 3-hydroxyacyl-ACP dehydratase component of the plant mitochondrial fatty acid ...

Plant Physiology (2017)

Xin Guan, Yozo Okazaki, Andrew Lithio, Ling Li, et al.

We report the characterization of the Arabidopsis 3-hydroxyacyl-acyl carrier protein (ACP) dehydratase (mtHD) component of the mitochondrial fatty acid synthase ...

Integrating metabolomics and transcriptomics data to discover a biocatalyst that ...

The Plant Journal (2016)

Ludmila Rizhsky, Huanan Jin, Michael R. Shepard, Harry W. Scott, et al.

The Echinacea genus is exemplary of over 30 plant families that produce a set of bioactive amides, called alkamides. The ...

Haploid differentiation in maize kernels based on fluorescence imaging

Plant Breeding (2016)

Brett W. Boote, Daniel J. Freppon, Gerald N. De La Fuente, Thomas Lubberstedt, et al.

A new fluorescence-based method for inbred haploid differentiation in maize kernels was developed by utilizing the R1-nj color marker in ...

AAE13 encodes a dual-localized malonyl-CoA synthetase that is crucial for ...

The Plant Journal (2016)

Xin Guan and Basil J. Nikolau

Malonyl-CoA is a key intermediate in a number of metabolic processes associated with its role as a substrate in acylation ...