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Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism
BMC Plant Biology
  • Wieslawa I. Mentzen, CRS4 Bioinformatics Laboratory
  • Jianling Peng, Iowa State University
  • Nick Ransom, Iowa State University
  • Basil Nikolau, Iowa State University
  • Eve Wurtele, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
1-1-2008
DOI
10.1186/1471-2229-8-76
Abstract
Background: Elucidating metabolic network structures and functions in multicellular organisms is an emerging goal of functional genomics. We describe the co-expression network of three core metabolic processes in the genetic model plant Arabidopsis thaliana: fatty acid biosynthesis, starch metabolism and amino acid (leucine) catabolism. Results: These co-expression networks form modules populated by genes coding for enzymes that represent the reactions generally considered to define each pathway. However, the modules also incorporate a wider set of genes that encode transporters, cofactor biosynthetic enzymes, precursor-producing enzymes, and regulatory molecules. We tested experimentally the hypothesis that one of the genes tightly co-expressed with starch metabolism module, a putative kinase AtPERK10, will have a role in this process. Indeed, knockout lines of AtPERK10 have an altered starch accumulation. In addition, the co-expression data define a novel hierarchical transcript-level structure associated with catabolism, in which genes performing smaller, more specific tasks appear to be recruited into higher-order modules with a broader catabolic function. Conclusion: Each of these core metabolic pathways is structured as a module of co-expressed transcripts that co-accumulate over a wide range of environmental and genetic perturbations and developmental stages, and represent an expanded set of macromolecules associated with the common task of supporting the functionality of each metabolic pathway. As experimentally demonstrated, co-expression analysis can provide a rich approach towards understanding gene function.
Comments

This is an article from BMC Plant Biology 8 (2008): 1, doi:10.1186/1471-2229-8-76. Posted with permission.

Rights
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Copyright Owner
Mentzen et al
Language
en
File Format
application/pdf
Citation Information
Wieslawa I. Mentzen, Jianling Peng, Nick Ransom, Basil Nikolau, et al.. "Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism" BMC Plant Biology Vol. 8 Iss. 76 (2008) p. 1 - 15
Available at: http://works.bepress.com/basil-nikolau/4/