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Translational regulation of light-induced ribulose 1,5-bisphosphate carboxylase gene expression in amaranth
Molecular and Cellular Biology (1986)
  • James O. Berry, University of Utah
  • Basil J Nikolau, The University Of Utah
  • John P. Carr, University of Utah
  • Daniel F. Klessig, Rutgers University - New Brunswick/Piscataway
The regulation of the genes encoding the large and small subunits of ribulose 1,5-bisphosphate carboxylase
was examined in amaranth cotyledons in response to changes in illumination. When dark-grown cotyledons
were transferred into light, synthesis of the large- and small-subunit polypeptides was initiated very rapidly,
before any increase in the levels of their corresponding mRNAs. Similarly, when light-grown cotyledons were
transferred to total darkness, synthesis of the large- and small-subunit proteins was rapidly depressed without
changes in mRNA levels for either subunit. In vitro translation or in vivo pulse-chase experiments indicated
that these apparent changes in protein synthesis were not due to alterations in the functionality of the mRNAs
or to protein turnover, respectively. These results, in combination with our previous studies, suggest that the
expression of ribulose 1,5-bisphosphate carboxylase genes can be adjusted rapidly at the translational level and
over a longer period through changes in mRNA accumulation.
Publication Date
Publisher Statement
This is an article from Molecular and Cellular Biology 6 (1986): 2347, doi:10.1128/MCB.6.7.2347
Posted with permission. Copyright 1986 American Society for Microbiology
Citation Information
James O. Berry, Basil J Nikolau, John P. Carr and Daniel F. Klessig. "Translational regulation of light-induced ribulose 1,5-bisphosphate carboxylase gene expression in amaranth" Molecular and Cellular Biology Vol. 6 Iss. 7 (1986) p. 2347 - 2353
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