Skip to main content
Article
High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver
Analytical Chemistry
  • Ji Hyun Jun, Iowa State University
  • Zhihong Song, Iowa State University
  • Zhenjiu Liu, Iowa State University
  • Basil Nikolau, Iowa State University
  • Edward S. Yeung, Iowa State University
  • Young Jin Lee, Iowa State University
Document Type
Article
Publication Version
Published Version
Publication Date
3-17-2010
DOI
10.1021/ac902990p
Abstract
High-spatial resolution and high-mass resolution techniques are developed and adopted for the mass spectrometric imaging of epicuticular lipids on the surface of Arabidopsis thaliana. Single cell level spatial resolution of ∼12 μm was achieved by reducing the laser beam size by using an optical fiber with 25 μm core diameter in a vacuum matrix-assisted laser desorption ionization-linear ion trap (vMALDI-LTQ) mass spectrometer and improved matrix application using an oscillating capillary nebulizer. Fine chemical images of a whole flower were visualized in this high spatial resolution showing substructure of an anther and single pollen grains at the stigma and anthers. The LTQ-Orbitrap with a MALDI ion source was adopted to achieve MS imaging in high mass resolution. Specifically, isobaric silver ion adducts of C29 alkane (m/z 515.3741) and C28 aldehyde (m/z 515.3377), indistinguishable in low-resolution LTQ, can now be clearly distinguished and their chemical images could be separately constructed. In the application to roots, the high spatial resolution allowed molecular MS imaging of secondary roots and the high mass resolution allowed direct identification of lipid metabolites on root surfaces.
Comments

Reprinted (adapted) with permission from Analytical Chemistry 82 (2010): 3255, doi: 10.1021/ac902990p. Copyright 2010 American Chemical Society.

Copyright Owner
American Chemical Society
Language
en
File Format
application/pdf
Citation Information
Ji Hyun Jun, Zhihong Song, Zhenjiu Liu, Basil Nikolau, et al.. "High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver" Analytical Chemistry Vol. 82 Iss. 8 (2010) p. 3255 - 3265
Available at: http://works.bepress.com/basil-nikolau/12/