The objective of the study is to measure antibody titers against Leptospira interrogans in serum and vitreous humour and determine the prevalence of L. interrogans in vitreous humour of horses with recurrent uveitis. 242 horses (270 eyes) with recurrent uveitis undergoing vitrectomy and 39 control horses (54 eyes) without any history or clinical signs of recurrent uveitis undergoing euthanasia or enucleation for unrelated reasons, were studied. Serum and vitreous humour were tested for antibodies against 13 serovars of L. interrogans. Vitreous humour was submitted for leptospiral culture; isolates were typed to the serogroup level. Leptospira interrogans was isolated from vitreous humour from 120/229 (52%) horses (126/252 [50%] eyes) with recurrent uveitis but was not isolated from vitreous humour from 36 eyes of 21 control horses. Duration of recurrent uveitis was 1 year for 45 of the 120 (38%) horses from which the organism was isolated. Geometric mean antibody titers against L. interrogans in the vitreous humour and serum of horses with recurrent uveitis were 1 : 1,332 and 1 : 186, respectively. Only 91 of 120 (76%) horses from which the organism was isolated had a 4-fold or greater difference between serum and vitreous humour antibody titers. Results suggest that persistent ocular infection with L. interrogans is common in horses with recurrent uveitis. A 4-fold increase in vitreous humour versus serum anti-body titers may not be a sensitive test for the diagnosis of L. interrogans-induced recurrent uveitis. We hypothesize that the immune component of recurrent uveitis can be directly induced and maintained by persistent infection of the eye with L. interrogans.
- antibodies infection uveitis horses Leptospira interrogans Equus Equidae Perissodactyla mammals vertebrates Chordata animals ungulates eukaryotes Leptospira Leptospiraceae Spirochaetales Gracilicutes bacteria prokaryotes
Available at: http://works.bepress.com/barton_rohrbach/94/