Identification, isolation and characterization of a human T cell population by a monoclonal antibodyCurrent Microbiology (1979)
AbstractThe hybridization of spleen cells from mice immunized with mononuclear leukocytes with the HAT-sensitive nonsecreting myeloma, NS1, resulted in the production of hybrid cell lines secreting monoclonal antibodies to lymphocyte surface antigens. One of these, anti-Ta, was shown by fluorescence-activated cell sorter analysis to be specific for a subpopulation of peripheral human T cells. Anti-Ta did not react with peripheral human B cells. Immunoprecipitation followed by two-dimensional gel analysis demonstrated that the T cell subpopulation-specific antigen recognized by this monoclonal antibody is part of, or firmly associated with, a protein of the plasma membrane.
Citation InformationBarbara A. Osborne. "Identification, isolation and characterization of a human T cell population by a monoclonal antibody" Current Microbiology Vol. 3 (1979)
Available at: http://works.bepress.com/barbara_osborne/11/