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Selected Works of Baoyu Chen
Article
Ena/VASP Proteins Cooperate with the WAVE Complex to Regulate the Actin Cytoskeleton
Developmental Cell (2014)
  • Xing Judy Chen, London Research Institute
  • Anna Julia Squarr, University of Münster
  • Raiko Stephan, University of Muenster
  • Baoyu Chen, UT Southwestern Medical Center
  • Theresa E. Higgins, London Research Institute
  • David J. Barry, London Research Institute
  • Morag C. Martin, London Research Institute
  • Mark K. Rosen, UT Southwestern Medical Center
  • Sven Bogdan, University of Muenster
  • Michael Way, London Research Institute
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Abstract
Ena/VASP proteins and the WAVE regulatory complex (WRC) regulate cell motilityby virtue of their ability to independently promote actin polymerization. We demonstrate that Ena/VASP and the WRC control actin polymerization in a cooperative manner through the interaction of the Ena/VASP EVH1 domain with an extended proline rich motif in Abi. This interaction increases cell migration and enables VASP to cooperatively enhance WRC stimulation of Arp2/3 complex-mediated actin assembly in vitro in the presence of Rac. Loss of this interaction in Drosophila macrophages results in defects in lamellipodia formation, cell spreading, and redistribution of Ena to the tips of filopodia-like extensions. Rescue experiments of abi mutants also reveals a physiological requirement for the Abi:Ena interaction in photoreceptor axon targeting and oogenesis. Our data demonstrate that the activities of Ena/VASP and the WRC are intimately linked to ensure optimal control of actin polymerization during cell migration and development.
Disciplines
Publication Date
September 8, 2014
DOI
10.1016/j.devcel.2014.08.001
Publisher Statement
Copyright 2014 The Authors
Citation Information
Xing Judy Chen, Anna Julia Squarr, Raiko Stephan, Baoyu Chen, et al.. "Ena/VASP Proteins Cooperate with the WAVE Complex to Regulate the Actin Cytoskeleton" Developmental Cell Vol. 30 Iss. 5 (2014) p. 569 - 584
Available at: http://works.bepress.com/baoyu-chen/4/
Creative Commons license
Creative Commons License
This work is licensed under a Creative Commons CC_BY International License.