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Design of a protective single-dose intranasal nanoparticle-based vaccine platform for respiratory infectious diseases
  • Bret Daniel Ulery, Iowa State University
  • Devender Kumar, Albany Medical College
  • Amanda Ellen Ramer-Tait, Iowa State University
  • Dennis W. Metzger, Albany Medical College
  • Michael J. Wannemuehler, Iowa State University
  • Balaji Narasimhan, Iowa State University
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Despite the successes provided by vaccination, many challenges still exist with respect to controlling new and re-emerging infectious diseases. Innovative vaccine platforms composed of adaptable adjuvants able to appropriately modulate immune responses, induce long-lived immunity in a single dose, and deliver immunogens in a safe and stable manner via multiple routes of administration are needed. This work describes the development of a novel biodegradable polyanhydride nanoparticle-based vaccine platform administered as a single intranasal dose that induced long-lived protective immunity against respiratory disease caused by Yesinia pestis, the causative agent of pneumonic plague. Relative to the responses induced by the recombinant protein F1-V alone and MPLA-adjuvanted F1-V, the nanoparticle-based vaccination regimen induced an immune response that was characterized by high titer and high avidity IgG1 anti-F1-V antibody that persisted for at least 23 weeks post-vaccination. After challenge, no Y. pestis were recovered from the lungs, livers, or spleens of mice vaccinated with the nanoparticle-based formulation and histopathological appearance of lung, liver, and splenic tissues from these mice post-vaccination was remarkably similar to uninfected control mice.

This article is from PLoS ONE 6 (2011): e17642, doi: 10.1371/journal.pone.0017642.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Ulery et al.
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Bret Daniel Ulery, Devender Kumar, Amanda Ellen Ramer-Tait, Dennis W. Metzger, et al.. "Design of a protective single-dose intranasal nanoparticle-based vaccine platform for respiratory infectious diseases" PLoS ONE Vol. 6 Iss. 3 (2011) p. article no. 17642
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