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Fluorescein-polyethyleneimine coated gadolinium oxide nanoparticles as T1 magnetic resonance imaging (MRI)–cell labeling (CL) dual agents
RSC Advances (2012)
  • Wenlong Xu, Kyungpook National University
  • Ja Young Park, Kyungpook National University
  • Krishna Kattel, Kyungpook National University
  • Md Wasi Ahmad, Kyungpook National University
  • Badrul Alam Bony, Kyungpook National University
  • Woo Choul Heo, Kyungpook National University
  • Seonguk Jin, Kyungpook National University
  • Jang Woo Park, Kyungpook National University
  • Yongmin Chang, Kyungpook National University
  • Tae Jeong Kim, Kyungpook National University
  • Ji Ae Park, Kyungpook National University
  • Ji Yeon Do, Kyungpook National University
  • Kwon Seok Chae, Kyungpook National University
  • Gang Ho Lee, Kyungpook National University
Abstract

We report the synthesis, characterization and application of highly water-soluble fluoresceinpolyethyleneimine (PEI) coated gadolinium oxide (Gd2O3) nanoparticles to magnetic resonance imaging (MRI) and cell labeling (CL). The average particle diameter and average hydrodynamic diameter were estimated to be 3.92 and 7.5 nm, respectively. Fluorescein-PEI was prepared from EDC/NHS coupling method. The surface coating was characterized by the FT-IR absorption spectrum and the surface coating amount was estimated to be 22.42 wt% from a TGA analysis, corresponding to 0.65 nm22 grafting density. The fluorescein-PEI coated gadolinium oxide nanoparticles showed r1 and r2 of 6.76 and 20.27 s21mM21, respectively, and a strong fluorescence at y527 nm. A pronounced positive contrast enhancement was clearly observed in 3 tesla T1 MR images of a rat with a liver tumor after injection of an aqueous sample solution into a rat tail vein. After treatment of DU145 cells with a sample solution, a strong fluorescence in confocal images was also observed. These two results together confirm the excellent MRI-CL dual functionality of fluorescein-PEI coated gadolinium oxide nanoparticles.

Publication Date
2012
Citation Information
Wenlong Xu, Ja Young Park, Krishna Kattel, Md Wasi Ahmad, et al.. "Fluorescein-polyethyleneimine coated gadolinium oxide nanoparticles as T1 magnetic resonance imaging (MRI)–cell labeling (CL) dual agents" RSC Advances Vol. 2 (2012)
Available at: http://works.bepress.com/badrul_bony/1/