Identity authentication systems such as biometrics can enable organizations to implement identity authentication and security with greater reliability and accountability. Adoption of these systems is a complex and costly endeavor and susceptible to failure when not aligned to the organization’s culture. We examine this adoption behavior utilizing the Unified Theory of Acceptance and Use of Technology (UTAUT) that provides a unified view of user acceptance. In this research, we investigate how espoused organizational culture values influence users’ acceptance and use of biometrics technology by considering cultural values as antecedents to the UTAUT model. We conducted an empirical study on the acceptance of a biometric identity authentication system and found that espoused organizational culture values have a significant effect on the UTAUT variables. Facilitating conditions, social influence, and anxiety affect the use of biometric identity authentication systems. Our research contributes to the literature on espoused organizational culture, biometrics, and technology acceptance.