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Article
Homology Modeling and In silico Analysis of COX from Channa punctata
Bioresearch Bulletin (2010)
  • Azhaguraj Ramakrishnan
Abstract
The Cytochrome c oxidase (COX) region of mitochondrial DNA is the most studied region of the fish mitochondrial genome. COX is one of the largest protein coding genes of metazoan mitochondrial genome.COX (E.C.1.9.3.1) is the terminal member of the respiratory chain catalyzing the reduction of dioxygen to water by ferrocytochrome C. A 19.927 kDa COX has been characterized in Channa punc-tata where COX III dimensional structure was generated using Deep View/ Swiss Pdp Viewer 3.7(Sps) by homol-ogy modeling, predicted model was validated in RAM-PAGE Server and COX secondary structure is predicted by PSIPRED, PHYRE and TNHMM Server. Protein statics was carried out by using the SAPS and CLC sequence viewer. The predicted 3-D model shows that most 95 % of residues have φ and ψ angle in the core and allowed re-gions, α-helix region 89.33%, β-sheet 9.33%, fully allowed regions 81.52%, additionally allowed regions 14.13%, generally allowed regions 3.26%, isoelectric point 4.95 and aliphatic index 121.72, from these results we can un-derstand the structure and properties of protein COX. However, homology modeling will give more insight on DNA bar coding.
Keywords
  • Homology modeling,
  • cytochrome c oxidase,
  • mitochondrial DNA,
  • barcoding
Disciplines
Publication Date
September 13, 2010
Citation Information
Azhaguraj Ramakrishnan. "Homology Modeling and In silico Analysis of COX from Channa punctata" Bioresearch Bulletin Vol. 1 Iss. 2 (2010)
Available at: http://works.bepress.com/azhaguraj_ramakrishnan/4/