Ethiopia is well known for its wide range of biological resources, both in terms of flora and faunal wealth. This region is not only rich in endemic species but also in native population of several useful or medicinally/commercially important plants and their relatives. In the recent past, a large number of commercially important tree species are over-exploited to meet the growing timber requirement of country, which is leading to the unseen environmental degradation, deforestation and several other undesirable effects (for example extinction of some plant species) on the biodiversity at initial stage. Moreover, it may also be directly or indirectly related with the food security, income, nutrition, employment and energy sources. Hence, a large-scale of plantation/aforestation of frequently used commercial tree species is urgently required to fulfill the demands and to restore the ecological balance. Under these circumstances plant tissue culture may appear to be the advantagous and perfect means of multiplying different kinds of endangered and commercially important tree species. This is a technique of growing plant cells, tissue and organs in a nutrient medium under aseptic and controlled environmental conditions. A variety of techniques are available, which can be used to get plant development in vitro. Shoot tip culture is the method in widest use for the mass propagation of woody species. If the phytohormones are appropriately balanced, the shoot tip elongates, lateral buds break and begin growth, and adventitious shoots are also produced on the stem piece. Shoots are removed from the cultures at regular intervals and a portion of the mass is replaced on fresh media to continue proliferation. Shoots produced through tissue culture are generally easy-to-root, even though the same cultivar may be difficult-to-root by cutting propagation. The present communication will highlight the importance of the technology and methodology in detail in order to share the knowledge; so that this technology can be used for large-scale plantation/reforestation programmes in Ethiopia.
Available at: http://works.bepress.com/azamal_husen/37/